Description
| Product Name: | Tartrate Resistant Acid Phosphatase (TRAP) Activity Assay Kit |
| Product Code: | MAES0271 |
| Sample Type: | Serum, Plasma, Urine,Animal Tissue And Cell Samples |
| Assay Time: | 25 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | TRAP |
| Detection Instrument: | Microplate reader (410-415 nm, optimum wavelength: 405 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 9.22 U/L |
| Detection Range: | 9.22-100 U/L |
| Inter-assay CV: | 0.023 |
| Intra-assay CV: | 0.02 |
The chromogenic agent can be catalyzed by acid phosphatase to produce p-nitrophenol in acidic condition. P-nitrophenol has a maximum absorption at 405 nm. The activity of TRAP can be calculated by measuring the amount of produced p-nitrophenol. The activity of acid phosphatase being detected in the presence of tartric acid is regarded as TRAP activity.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
| Other Instruments Required: | Incubator(37℃) |