Name: TAF4B Antibody (PACO59317)
SKU: PACO59317
Availability: OutOfStock

Antibody Name:	TAF4B Antibody (PACO59317)
Antibody SKU:	PACO59317
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Transcription initiation factor TFIID subunit 4B protein (132-244AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO59317 diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with PACO59317 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.

Synonyms:

Transcription initiation factor TFIID subunit 4B (Transcription initiation factor TFIID 105 kDa subunit) (TAF(II)105) (TAFII-105) (TAFII105), TAF4B, TAF2C2 TAFII105

UniProt Protein Function:	TAF4B: Cell type-specific subunit of TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAFII105 is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Belongs to the TAF4 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 18q11.2 Cellular Component: nucleolus; nucleoplasm Molecular Function:DNA binding; NF-kappaB binding Biological Process: RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter Disease: Spermatogenic Failure 13
NCBI Summary:	TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
UniProt Code:	Q92750
NCBI GenInfo Identifier:	119370533
NCBI Gene ID:	6875
NCBI Accession:	Q92750.2
UniProt Secondary Accession:	Q92750,Q29YA4, Q29YA5,
UniProt Related Accession:	Q92750
Molecular Weight:	63,364 Da
NCBI Full Name:	Transcription initiation factor TFIID subunit 4B
NCBI Synonym Full Names:	TATA-box binding protein associated factor 4b
NCBI Official Symbol:	TAF4B
NCBI Official Synonym Symbols:	SPGF13; TAF2C2; TAFII105
NCBI Protein Information:	transcription initiation factor TFIID subunit 4B
UniProt Protein Name:	Transcription initiation factor TFIID subunit 4B
UniProt Synonym Protein Names:	Transcription initiation factor TFIID 105 kDa subunit; TAF(II)105; TAFII-105; TAFII105
Protein Family:	Transcription initiation factor
UniProt Gene Name:	TAF4B
UniProt Entry Name:	TAF4B_HUMAN