Enzymes Recombinant Proteins

T4 DNA Recombinant Protein (RPPB2404)

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RPPB2404

Description

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Product Name:T4 DNA Recombinant Protein
Product Code:RPPB2404
Size:100,000IU
Target:T4 DNA
Synonyms:DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.
Source:Escherichia Colilambda lysogen NM 989
Physical Appearance:Sterile filtered liquid formulation having a concentration of 167,000 U/ml.
Formulation:50mM Tris-HCl (pH 7.8 at 25�C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 �g/ml BSA and DNA (0.1 to 1 �m in 5� termini). Optimal ligation occurs at 16C.
Biological Activity:One Weiss unit is equivalent to circa 67 cohesive-end ligation units.� T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.� Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50�M.� To dilute T4 DNA Ligase that will subsequently be stored at �20�C, 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

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Additional Information

Product Type:
Recombinant Protein
Research Area:
Enzymes
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