Enzymes Recombinant Proteins
T4 DNA Recombinant Protein (RPPB2404)
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- SKU:
- RPPB2404
- Product Type:
- Recombinant Protein
- Research Area:
- Enzymes
Description
| Product Name: | T4 DNA Recombinant Protein |
| Product Code: | RPPB2404 |
| Size: | 100,000IU |
| Target: | T4 DNA |
| Synonyms: | DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4. |
| Source: | Escherichia Colilambda lysogen NM 989 |
| Physical Appearance: | Sterile filtered liquid formulation having a concentration of 167,000 U/ml. |
| Formulation: | 50mM Tris-HCl (pH 7.8 at 25�C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 �g/ml BSA and DNA (0.1 to 1 �m in 5� termini). Optimal ligation occurs at 16C. |
| Biological Activity: | One Weiss unit is equivalent to circa 67 cohesive-end ligation units.� T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.� Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50�M.� To dilute T4 DNA Ligase that will subsequently be stored at �20�C, 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used. |
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
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