Hormone & Small Molecule ELISA Kits

T3 / Triiodothyronine ELISA Kit

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SKU:
UNFI0016
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Competitive
Synonyms:
T3, Triiodothyronine
Reactivity:
Universal

Description

T3 / Triiodothyronine ELISA Kit

The T3 ELISA Kit is designed for the quantitative measurement of triiodothyronine (T3) levels in biological samples. T3 is a vital thyroid hormone involved in metabolism, growth, and other physiological processes. This kit allows researchers to accurately assess T3 concentrations, aiding studies related to thyroid function, metabolism, and hormonal regulation.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

T3 (Triiodothyronine) ELISA Kit

Product Code:

UNFI0016

Size:

96 Assays

Alias:

T3, Triiodothyronine

Detection Method:

Competitive ELISA, Coated with Antibody

Reactivity:

Universal

Sensitivity:

0.094ng/ml

Range:

0.156-10ng/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of T3 and the recovery rates were calculated by comparing the measured value to the expected amount of T3 in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

92-101

97

EDTA plasma (n=5)

85-105

99

UFH plasma (n=5)

89-94

92

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of T3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

87-100%

90-104%

85-99%

EDTA plasma (n=5)

85-97%

83-101%

82-99%

UFH Plasma (n=5)

80-90%

83-99%

85-100%

Intra Assay

CV < 8%

Inter Assay

CV < 10%

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

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