SYNE2 Antibody (PACO58352)
- SKU:
- PACO58352
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SYNE2 Antibody (PACO58352) |
| Antibody SKU: | PACO58352 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Nesprin-2 protein (1-176AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human endometrial cancer using PACO58352 at dilution of 1:100. |
 | Immunofluorescent analysis of MCF-7 cells using PACO58352 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. May be involved in nucleus-centrososme attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probable association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 may act redundantly. During INM at G1 phase mediates respective LINC complex association with kinesin to push the nucleus away from the centrosome. Involved in nuclear migration in retinal photoreceptor progenitors. Required for centrosome migration to the apical cell surface during early ciliogenesis. |
| Synonyms: | Nesprin-2 (KASH domain-containing protein 2) (KASH2) (Nuclear envelope spectrin repeat protein 2) (Nucleus and actin connecting element protein) (Protein NUANCE) (Synaptic nuclear envelope protein 2) (Syne-2), SYNE2, KIAA1011 NUA |
| UniProt Protein Function: | SYNE2: a nuclear transmembrane protein involved in the maintenance of nuclear organization and structural integrity. The largest part of the protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells. Probable anchoring protein which theters the nucleus to the cytoskeleton. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Nine alternatively spliced isoforms have been described.Protein type: Membrane protein, integral; Adaptor/scaffoldChromosomal Location of Human Ortholog: 14q23.2Cellular Component: nucleoplasm; filopodium membrane; nuclear outer membrane; intermediate filament cytoskeleton; nuclear membrane; sarcoplasmic reticulum membrane; nuclear lumen; focal adhesion; sarcoplasmic reticulum; mitochondrion; cytoplasm; integral to membrane; nuclear envelope; nucleus; Z discMolecular Function: actin filament binding; protein binding; actin bindingBiological Process: centrosome localization; establishment and/or maintenance of cell polarity; nuclear migration along microfilament; nuclear migration; nuclear membrane organization and biogenesis; positive regulation of cell migrationDisease: Emery-dreifuss Muscular Dystrophy 5, Autosomal Dominant |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| UniProt Code: | Q8WXH0 |
| NCBI GenInfo Identifier: | 33624861 |
| NCBI Gene ID: | 23224 |
| NCBI Accession: | NP_878914.1 |
| UniProt Secondary Accession: | Q8WXH0,Q540G1, Q86YP9, Q8N1S3, Q8NF49, Q8TER7, Q8WWW3 Q8WWW4, Q8WWW5, B4DND7 |
| UniProt Related Accession: | Q8WXH0 |
| Molecular Weight: | |
| NCBI Full Name: | nesprin-2 isoform 2 |
| NCBI Synonym Full Names: | spectrin repeat containing, nuclear envelope 2 |
| NCBI Official Symbol: | SYNE2 |
| NCBI Official Synonym Symbols: | NUA; EDMD5; Nesp2; TROPH; NUANCE; SYNE-2; Nesprin-2 |
| NCBI Protein Information: | nesprin-2; nesprin 2; nuclear envelope spectrin repeat protein 2; nucleus and actin connecting element protein; polytrophin; synaptic nuclear envelope protein 2; synaptic nuclei expressed gene 2 |
| UniProt Protein Name: | Nesprin-2 |
| UniProt Synonym Protein Names: | Nuclear envelope spectrin repeat protein 2; Nucleus and actin connecting element protein; Protein NUANCE; Synaptic nuclear envelope protein 2; Syne-2 |
| Protein Family: | Nesprin |
| UniProt Gene Name: | SYNE2 |
| UniProt Entry Name: | SYNE2_HUMAN |
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