Cell Health Assays

Superoxide Dismutase (SOD) Activity Assay Kit (CV0015)

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SKU:
CV0015
Product Type:
Assay
Research Area:
Metabolism

Description

ELISA Kit Technical Manual

Superoxide Dismutase (SOD) Activity Assay Kit

Superoxide dismutases (SOD) catalyse the breakdown of superoxide radicals and provide the first line of defense against oxygen toxicity.

SOD Assay Kit, superoxide ions are generated from the conversion of xanthine and O2 to uric acid and H2O2 by Xanthine Oxidase (XO). The superoxide anion then coverts the tetrazolium salt WST-1 to the colored product WST-1 formazan.

Absorbance is then measured at 450 nm using a standard microplate reader. Addition of SOD to this reaction reduces superoxide ion levels, thereby lowering the rate of WST-1 formazan formation. SOD activity in the experimental sample is measured as the percent inhibition of the rate of WST-1 formazan formation.

Kit Information:

Overview Description

Size:

100 Assays

Featutes & Benefits:
  • Easy-to-use.
  • Fast protocol.

Kit Contents:
  • 1 mL WST-1 reagent
  • 20 mL SOD Assay Buffer
  • 10 mL SOD Dilution Buffer
  • 30 μL SOD Enzyme solution
  • 1 mg SOD Standard (300 U/mg)

Applications:

Quantitative determination of Superoxide Dismutase (SOD) enzyme activity.

Shipping & Storage:
  • Shipping: Gel pack.
  • Storage: The SOD Standard should be stored at -20 °C. All other components of this kit are stable at 4 °C until the kit expiration date.
  • Shelf Life: 24 months from date of purchase, if stored properly.
  • Recommendations: WST-1 reagent is light sensitive and should be maintained in amber tubes.

Safety:

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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