Superoxide Anionic Assay Kit - Colorimetric (WST-1 Method) (MAES0014)
- SKU:
- MAES0014
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Urine, Cells, Cell Culture Supernatant, Leucocyte
- Assay Time:
- 35 min
- Shipping:
- Gel Pack
- Research Area:
- Oxidative Stress
- Research Area:
- Reactive Oxygen Species
Description
| Product Name: | Superoxide Anionic Assay Kit - Colorimetric (WST-1 Method) |
| Product Code: | MAES0014 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, urine, cells, cell culture supernatant, leucocyte |
| Assay Time: | 35 min |
| Instrument: | Microplate reader |
| Recovery Rate: | 100 |
| Inter CV: | 5.8 |
| Inter CV: | 2 |
Superoxide anion radical is a kind of reactive oxygen, which is formed by the reduction of molecular oxygen. Excessive accumulation of reactive oxygen species will lead to oxidative stress.
Superoxide anion free radicals are produced through the reaction system of xanthine and xanthine oxidase. WST-1 (a water-soluble tetrazolium salt) can react with the generated superoxide anion to produce water-soluble formazan. When the tested sample contains the superoxide anion free radical inhibitor, it can inhibit the formation of formazan. When the tested sample contains the substance that produces superoxide anion free radical, it can promote the formation of formazan dye. By colorimetric analysis of WST-1 products, the units of activity of inhibition or production of superoxide anion radical in samples can be calculated.
| Shelf Life: | 6 Months |
| Storage: | Reagent 3:-20°C;, Others:2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Oxidative stress |
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