Succinate Dehydrogenase (SDH) Activity Assay Kit

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SKU:
MAES0229
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Animal Tissue, Cell
Sensitivity:
0.83 U/L
Range:
0.83-65.42 U/L
Assay Time:
10 min

Description

Product Name:Succinate Dehydrogenase (SDH) Activity Assay Kit
Product Code:MAES0229
Sample Type:Animal Tissue, Cell
Assay Time:10 min
Assay Type:Enzyme Activity
Synonyms:SDH
Detection Instrument:Microplate reader (590-610 nm, optimum wavelength: 600 nm)
Detection Method:Colorimetric method
Sensitivity:0.83 U/L
Detection Range:0.83-65.42 U/L
Inter-assay CV:0.04
Intra-assay CV:0.02

SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP). Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm.

Storage:-20°C
Valid Period:12 months
Other Reagents Required:Normal saline (0.9% NaCl), PBS(0.01 M，pH 7.4).
Other Instruments Required:Centrifuge
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