Streptavidin Protein Recombinant Protein (RPPB5044)
- SKU:
- RPPB5044
Description
| Product Name: | Streptavidin Protein Recombinant Protein |
| Product Code: | RPPB5044 |
| Size: | 10mg |
| Target: | Streptavidin Protein |
| Source: | Bacterium Streptomyces avidinii |
| Physical Appearance: | Sterile Filtered lyophilized powder. |
| Formulation: | The Streptavidin was lyophilized from a 25mg/ml solution in 10 mM potassium phosphate buffer pH 6.5 |
| Solubility: | Gives a clear solution at 5mg/ml in 0.1M NaCl. |
| Stability: | Streptavidin although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter.Please prevent freeze-thaw cycles. |
Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
Streptavidin is a protein produced by Streptomyces avidinii and isolated by purification from fermentation broth. The pure, homogeneous protein shows predominantly one single band in SDS PAGE. Streptavidin consists of 4 identical subunits, each bearing an active binding site for biotin. Streptavidin has a molecular weight of 55kDa.
| UniProt Protein Function: | streptavidin: The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). (organism: Streptomyces avidinii) |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P22629 |
| NCBI GenInfo Identifier: | 134951 |
| NCBI Gene ID: | |
| NCBI Accession: | P22629.1 |
| UniProt Secondary Accession: | P22629 |
| UniProt Related Accession: | P22629 |
| Molecular Weight: | |
| NCBI Full Name: | Streptavidin |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | |
| UniProt Protein Name: | Streptavidin |
| UniProt Synonym Protein Names: | |
| Protein Family: | Streptavidin |
| UniProt Gene Name: | |
| UniProt Entry Name: | SAV_STRAV |
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