Description
Streptavidin-HRP Information
| Product Sku | KESAP002 |
| Quantity | 1 mL |
| Storage | 2-8°C |
| Stability | 12 months |
| Description | Enzymatic reagent to react with biotinylated detection antibody in ELISA. |
| Preparation | Dilute 500 µL of Streptavidin-HRP in 11.5 mL of 4% BSA in DPBS, or other acceptable diluent, for use in ELISA. |
Streptavidin-HRP Procedure
| 1. | After Biotinylated Detection Antibody incubation, wash plate FOUR times with Wash Buffer. |
| 2. | Prepare Streptavidin-HRP Working Solution as described. |
| 3. | Add 100 µL of Streptavidin-HRP Working Solution to each well. |
| 4. | Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. |
| 5. | Wash plate FOUR times with Wash Buffer and proceed with HRP Substrate. ELISA Accessory Pack: Product Sku: KESAP001 |
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