Name: STAG3 Antibody (PACO56366)
SKU: PACO56366
Availability: OutOfStock

Antibody Name:	STAG3 Antibody (PACO56366)
Antibody SKU:	PACO56366
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Cohesin subunit SA-3 protein (1056-1212AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO56366 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of PACO56366 diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I.

Synonyms:

Cohesin subunit SA-3 (SCC3 homolog 3) (Stromal antigen 3) (Stromalin-3), STAG3

UniProt Protein Function:	STAG3: Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis- specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I. Belongs to the SCC3 family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 7q22.1Cellular Component: chromosome, pericentric region; extracellular space; nucleus; synaptonemal complexBiological Process: synaptonemal complex assemblyDisease: Premature Ovarian Failure 8
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene is expressed in the nucleus and is a subunit of the cohesin complex which regulates the cohesion of sister chromatids during cell division. A mutation in this gene is associated with premature ovarian failure. Alternate splicing results in multiple transcript variants encoding distinct isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Apr 2014]
UniProt Code:	Q9UJ98
NCBI GenInfo Identifier:	68847235
NCBI Gene ID:	10734
NCBI Accession:	Q9UJ98.2
UniProt Secondary Accession:	Q9UJ98,Q8NDP3, A6H8Z1, B4DZ10, D6W5U8, H7BYK9
UniProt Related Accession:	Q9UJ98
Molecular Weight:	132,319 Da
NCBI Full Name:	Cohesin subunit SA-3
NCBI Synonym Full Names:	stromal antigen 3
NCBI Official Symbol:	STAG3
NCBI Official Synonym Symbols:
NCBI Protein Information:	cohesin subunit SA-3
UniProt Protein Name:	Cohesin subunit SA-3
UniProt Synonym Protein Names:	SCC3 homolog 3; Stromal antigen 3; Stromalin-3
Protein Family:	STAG3-like protein
UniProt Gene Name:	STAG3
UniProt Entry Name:	STAG3_HUMAN