Antibody Name:	SSX2IP Antibody (PACO56270)
Antibody SKU:	PACO56270
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human, Rat
Immunogen:	Recombinant Human Afadin- and alpha-actinin-binding protein (485-614AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, Rat liver tissue. All lanes: SSX2IP antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 72, 66, 69 kDa. Observed band size: 72 kDa.
	IHC image of PACO56270 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO56270 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome. Involved in ciliogenesis. It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia.

Synonyms:

Afadin- and alpha-actinin-binding protein (ADIP) (Afadin DIL domain-interacting protein) (SSX2-interacting protein), SSX2IP, KIAA0923

UniProt Protein Function:	SSX2IP: Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E- cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Cell adhesionChromosomal Location of Human Ortholog: 1p22.3Cellular Component: protein complex; cell-cell adherens junction; leading edge; nucleusMolecular Function: protein domain specific binding; protein bindingBiological Process: regulation of Rac protein signal transduction; centrosome organization and biogenesis; cilium biogenesis; cell adhesion
UniProt Protein Details:
NCBI Summary:	This gene encodes a protein that binds the cancer-testis antigen Synovial Sarcoma X breakpoint 2 protein. The encoded protein may regulate the activity of Synovial Sarcoma X breakpoint 2 protein in malignant cells. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Oct 2009]
UniProt Code:	Q9Y2D8
NCBI GenInfo Identifier:	261878552
NCBI Gene ID:	117178
NCBI Accession:	NP_001159889.1
UniProt Secondary Accession:	Q9Y2D8,Q6P2P8, Q6ULS1, Q7L168, Q9UIX0, A8K8W0, B4DFE3 D3DT13, J3KR02
UniProt Related Accession:	Q9Y2D8
Molecular Weight:	68,274 Da
NCBI Full Name:	afadin- and alpha-actinin-binding protein isoform 1
NCBI Synonym Full Names:	synovial sarcoma, X breakpoint 2 interacting protein
NCBI Official Symbol:	SSX2IP
NCBI Official Synonym Symbols:	ADIP
NCBI Protein Information:	afadin- and alpha-actinin-binding protein; SSX2-interacting protein; afadin DIL domain-interacting protein
UniProt Protein Name:	Afadin- and alpha-actinin-binding protein
UniProt Synonym Protein Names:	Afadin DIL domain-interacting protein; SSX2-interacting protein
Protein Family:	Afadin- and alpha-actinin-binding protein
UniProt Gene Name:	SSX2IP
UniProt Entry Name:	ADIP_HUMAN