SSU72 Antibody (PACO40622)
- SKU:
- PACO40622
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SSU72 Antibody (PACO40622) |
| Antibody SKU: | PACO40622 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human RNA polymerase II subunit A C-terminal domain phosphatase SSU72 protein (1-194AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of PC-3 cells with PACO40622 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK. |
| Synonyms: | RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (CTD phosphatase SSU72) (EC 3.1.3.16), SSU72 |
| UniProt Protein Function: | SSU72: May be involved in the C-terminal domain of RNA polymerase II dephosphorylation, RNA processing and termination. Belongs to the SSU72 phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Phosphatase; EC 3.1.3.16Chromosomal Location of Human Ortholog: 1p36.33Cellular Component: cytoplasm; nucleusMolecular Function: protein binding; CTD phosphatase activityBiological Process: mRNA polyadenylation |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9NP77 |
| NCBI GenInfo Identifier: | 74752877 |
| NCBI Gene ID: | 29101 |
| NCBI Accession: | Q9NP77.1 |
| UniProt Secondary Accession: | Q9NP77,Q9BZS6, Q9H933 |
| UniProt Related Accession: | Q9NP77 |
| Molecular Weight: | 16,931 Da |
| NCBI Full Name: | RNA polymerase II subunit A C-terminal domain phosphatase SSU72 |
| NCBI Synonym Full Names: | SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae) |
| NCBI Official Symbol: | SSU72 |
| NCBI Official Synonym Symbols: | HSPC182; PNAS-120 |
| NCBI Protein Information: | RNA polymerase II subunit A C-terminal domain phosphatase SSU72; CTD phosphatase SSU72 |
| UniProt Protein Name: | RNA polymerase II subunit A C-terminal domain phosphatase SSU72 |
| UniProt Synonym Protein Names: | |
| Protein Family: | RNA polymerase II subunit A C-terminal domain phosphatase |
| UniProt Gene Name: | SSU72 |
| UniProt Entry Name: | SSU72_HUMAN |
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