Name: SSH1 Antibody (PACO60248)
SKU: PACO60248
Availability: OutOfStock

Antibody Name:	SSH1 Antibody (PACO60248)
Antibody SKU:	PACO60248
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Protein phosphatase Slingshot homolog 1 protein (928-1049AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO60248 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with PACO60248 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:	Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
Synonyms:	Protein phosphatase Slingshot homolog 1 (EC 3.1.3.16) (EC 3.1.3.48) (SSH-like protein 1) (SSH-1L) (hSSH-1L), SSH1, KIAA1298 SSH1L

UniProt Protein Function:	SSH1: a dual-specificity phosphatase of the Slingshot family involved in actin reorganization. Its calcineurin-dependent activation dephosphorylates cofilin. Plays a role in later stages of mitosis by dephosphorylating and activating cofilin.Protein type: EC 3.1.3.16; Protein phosphatase, dual-specificity; EC 3.1.3.48Chromosomal Location of Human Ortholog: 12q24.11Cellular Component: cytoplasm; nucleoplasm; plasma membraneMolecular Function: actin binding; phosphoprotein phosphatase activity; protein bindingBiological Process: actin cytoskeleton organization and biogenesis; cell morphogenesis; protein amino acid dephosphorylation; regulation of actin polymerization and/or depolymerization; regulation of axonogenesis; regulation of cellular protein metabolic process
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:	Q8WYL5
NCBI GenInfo Identifier:	82582267
NCBI Gene ID:	54434
NCBI Accession:	Q8WYL5.2
UniProt Secondary Accession:	Q8WYL5,Q6P6C0, Q8N9A7, Q8WYL3, Q8WYL4, Q9P2P8
UniProt Related Accession:	Q8WYL5
Molecular Weight:	78,978 Da
NCBI Full Name:	Protein phosphatase Slingshot homolog 1
NCBI Synonym Full Names:	slingshot protein phosphatase 1
NCBI Official Symbol:	SSH1
NCBI Official Synonym Symbols:	SSH1L
NCBI Protein Information:	protein phosphatase Slingshot homolog 1
UniProt Protein Name:	Protein phosphatase Slingshot homolog 1
UniProt Synonym Protein Names:	SSH-like protein 1; SSH-1L; hSSH-1L
Protein Family:	Sec sixty-one protein
UniProt Gene Name:	SSH1
UniProt Entry Name:	SSH1_HUMAN