SPTLC3 Antibody (PACO40658)
- SKU:
- PACO40658
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SPTLC3 Antibody (PACO40658) |
| Antibody SKU: | PACO40658 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human Serine palmitoyltransferase 3 protein (1-58AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: SPTLC3 antibody at 0.2µg/ml. Lane 1: Mouse kidney tissue. Lane 2: U87 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 63, 20 kDa. Observed band size: 63 kDa. |
 | Immunohistochemistry of paraffin-embedded human colon cancer using PACO40658 at dilution of 1:100. |
 | Immunofluorescent analysis of HepG2 cells using PACO40658 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference. |
| Synonyms: | Serine palmitoyltransferase 3 (EC 2.3.1.50) (Long chain base biosynthesis protein 2b) (LCB2b) (Long chain base biosynthesis protein 3) (LCB 3) (Serine-palmitoyl-CoA transferase 3) (SPT 3), SPTLC3, C20orf38 SPTLC2L |
| UniProt Protein Function: | SPTLC3: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SSSPTB has the ability to use a broader range of acyl-CoAs without apparent preference. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 2.3.1.50; Endoplasmic reticulum; Transferase; Membrane protein, integralChromosomal Location of Human Ortholog: 20p12.1Cellular Component: endoplasmic reticulum membraneMolecular Function: serine C-palmitoyltransferase activityBiological Process: sphingoid biosynthetic process; sphingolipid biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | The SPTLC3 gene encodes an isoform of the third subunit of serine palmitoyltransferase (SPT; EC 2.3.1.50), which catalyzes the rate-limiting step of the de novo synthesis of sphingolipids (Hornemann et al., 2006 [PubMed 17023427]). SPT contains 2 main subunits: the common SPTLC1 subunit (MIM 605712) and either SPTLC2 (MIM 605713) or its isoform SPTLC2L (SPTLC3), depending on the tissue in which biosynthesis occurs (Hornemann et al., 2006 [PubMed 17023427]). There are also 2 highly related isoforms of a third subunit, SSSPTA (MIM 613540) and SSSPTB (MIM 610412), that confer acyl-CoA preference of the SPT enzyme and are essential for maximal enzyme activity (Han et al., 2009 [PubMed 19416851]).[supplied by OMIM, Nov 2010] |
| UniProt Code: | Q9NUV7 |
| NCBI GenInfo Identifier: | 158931158 |
| NCBI Gene ID: | 55304 |
| NCBI Accession: | Q9NUV7.3 |
| UniProt Secondary Accession: | Q9NUV7,Q05DQ8, Q5T1U4, Q9H1L1, Q9H1Z0, A2A2I4, B9EK64 |
| UniProt Related Accession: | Q9NUV7 |
| Molecular Weight: | 19,693 Da |
| NCBI Full Name: | Serine palmitoyltransferase 3 |
| NCBI Synonym Full Names: | serine palmitoyltransferase long chain base subunit 3 |
| NCBI Official Symbol: | SPTLC3 |
| NCBI Official Synonym Symbols: | LCB3; SPT3; LCB2B; hLCB2b; SPTLC2L; C20orf38; dJ718P11; dJ718P11.1 |
| NCBI Protein Information: | serine palmitoyltransferase 3 |
| UniProt Protein Name: | Serine palmitoyltransferase 3 |
| UniProt Synonym Protein Names: | Long chain base biosynthesis protein 2b; LCB2b; Long chain base biosynthesis protein 3; LCB 3; Serine-palmitoyl-CoA transferase 3; SPT 3 |
| Protein Family: | Serine palmitoyltransferase |
| UniProt Gene Name: | SPTLC3 |
| UniProt Entry Name: | SPTC3_HUMAN |
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