SPPL3 Antibody (PACO48706)
- SKU:
- PACO48706
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SPPL3 Antibody (PACO48706) |
| Antibody SKU: | PACO48706 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:200-1:2000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Signal peptide peptidase-like 3 protein (213-263AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescent analysis of HepG2 cells using PACO48706 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate. All lanes: SPPL3 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 19 kDa. Observed band size: 43 kDa. |
| Background: | Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries. Acts like a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1. Catalyzes the intramembrane cleavage of the envelope glycoprotein gp130 and/or the leader peptide gp18LP of the simian foamy virus independent of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis. May also have the ability to serve as a shedding protease for subsequent intramembrane proteolysis by SPPL2A and SPPL2B of the envelope glycoprotein gp130. Plays a role in the regulation of cellular glycosylation processes. Required to link T-cell antigen receptor (TCR) and calcineurin-NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease-independent manner. |
| Synonyms: | Signal peptide peptidase-like 3 (SPP-like 3) (EC 3.4.23) (Intramembrane protease 2) (IMP-2) (Presenilin homologous protein 1) (PSH1) (Presenilin-like protein 4), SPPL3, IMP2 PSL4 |
| UniProt Protein Function: | SPPL3: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Belongs to the peptidase A22B family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, multi-pass; EC 3.4.23.-; Protease; Membrane protein, integralChromosomal Location of Human Ortholog: 12q24.31Cellular Component: rough endoplasmic reticulumMolecular Function: protein homodimerization activity; aspartic endopeptidase activity, intramembrane cleavingBiological Process: membrane protein ectodomain proteolysis |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q8TCT6 |
| NCBI GenInfo Identifier: | 33413418 |
| NCBI Gene ID: | 121665 |
| NCBI Accession: | NP_620584.2 |
| UniProt Secondary Accession: | Q8TCT6 |
| UniProt Related Accession: | Q8TCT6,Q3MJ04, Q53EP9, Q6UWJ2, Q8TCT6, Q9UG23 |
| Molecular Weight: | 42,261 Da |
| NCBI Full Name: | signal peptide peptidase-like 3 |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | SPPL3 |
| NCBI Official Synonym Symbols: | IMP2; PSL4; PRO4332; MDHV1887; MGC90402; MGC126674; MGC126676; DKFZp586C1324 |
| NCBI Protein Information: | signal peptide peptidase-like 3; SPP-like 3; OTTHUMP00000240480; intramembrane protease 2; presenilin-like protein 4 |
| UniProt Protein Name: | Signal peptide peptidase-like 3 |
| UniProt Synonym Protein Names: | Intramembrane protease 2 |
| Protein Family: | Signal peptide peptidase |
| UniProt Gene Name: | SPPL3 |
| UniProt Entry Name: | SPPL3_HUMAN |
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