SPPL2B Antibody (PACO60232)
- SKU:
- PACO60232
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SPPL2B Antibody (PACO60232) |
| Antibody SKU: | PACO60232 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Signal peptide peptidase-like 2B protein (492-592AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of A549 cells with PACO60232 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-α (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity. Catalyzes the intramembrane cleavage of the simian foamy virus processed leader peptide gp18 of the envelope glycoprotein gp130 dependently of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis. |
| Synonyms: | Signal peptide peptidase-like 2B (SPP-like 2B) (SPPL2b) (EC 3.4.23) (Intramembrane protease 4) (IMP-4) (Presenilin homologous protein 4) (PSH4) (Presenilin-like protein 1), SPPL2B, IMP4 KIAA1532 PSL1 |
| UniProt Protein Function: | SPPL2B: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF- alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity. Belongs to the peptidase A22B family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Protease; EC 3.4.23.-; Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 19p13.3Cellular Component: actin cytoskeleton; centrosome; endosome membrane; lysosomal membrane; membrane; nucleoplasm; plasma membraneMolecular Function: aspartic endopeptidase activity, intramembrane cleaving; protein binding; protein homodimerization activityBiological Process: membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; membrane protein proteolysis; regulation of immune response; signal peptide processing |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the GXGD family of aspartic proteases. The GXGD proteases are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions. This enzyme localizes to endosomes, lysosomes, and the plasma membrane. It cleaves the transmembrane domain of tumor necrosis factor alpha to release the intracellular domain, which triggers cytokine expression in the innate and adaptive immunity pathways. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8TCT7 |
| NCBI GenInfo Identifier: | 97537015 |
| NCBI Gene ID: | 56928 |
| NCBI Accession: | Q8TCT7.2 |
| UniProt Secondary Accession: | Q8TCT7,O60365, Q567S3, Q8IUH9, Q9BUY6, Q9H3M4, Q9NPN2 Q9P1Z6, D6W609 |
| UniProt Related Accession: | Q8TCT7 |
| Molecular Weight: | 56,431 Da |
| NCBI Full Name: | Signal peptide peptidase-like 2B |
| NCBI Synonym Full Names: | signal peptide peptidase like 2B |
| NCBI Official Symbol: | SPPL2B |
| NCBI Official Synonym Symbols: | IMP4; PSH4; PSL1; IMP-4 |
| NCBI Protein Information: | signal peptide peptidase-like 2B |
| UniProt Protein Name: | Signal peptide peptidase-like 2B |
| UniProt Synonym Protein Names: | Intramembrane protease 4 |
| Protein Family: | Signal peptide peptidase |
| UniProt Gene Name: | SPPL2B |
| UniProt Entry Name: | SPP2B_HUMAN |
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