Antibody Name:	SPAG4 Antibody (PACO18426)
Antibody SKU:	PACO18426
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:50-1:200
Species Reactivity:	Human
Immunogen:	Synthetic peptide of human SPAG4

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO18426(SPAG4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
	Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hela cells, K562 cells, Primary antibody: PACO18426(SPAG4 Antibody) at dilution 1/1100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds.
	The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO18426(SPAG4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.

Synonyms:

Sperm associated antigen 4

UniProt Protein Function:	Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.
UniProt Protein Details:
NCBI Summary:	The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
UniProt Code:	Q9NPE6
NCBI GenInfo Identifier:	11037055
NCBI Gene ID:	6676
NCBI Accession:	NP_003107.1
UniProt Secondary Accession:	Q9NPE6,O43648
UniProt Related Accession:	Q9NPE6
Molecular Weight:	48,165 Da
NCBI Full Name:	sperm-associated antigen 4 protein isoform 1
NCBI Synonym Full Names:	sperm associated antigen 4
NCBI Official Symbol:	SPAG4
NCBI Official Synonym Symbols:	SUN4; CT127
NCBI Protein Information:	sperm-associated antigen 4 protein
UniProt Protein Name:	Sperm-associated antigen 4 protein
UniProt Synonym Protein Names:	Outer dense fiber-associated protein SPAG4; SUN domain-containing protein 4
Protein Family:	Sperm-associated antigen 4 protein
UniProt Gene Name:	SPAG4
UniProt Entry Name: