Antibody Name:	SPAG1 Antibody (PACO18425)
Antibody SKU:	PACO18425
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:1000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100
Species Reactivity:	Human
Immunogen:	Synthetic peptide of human SPAG1

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO18425(SPAG1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200).
	Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human colon tissue, Primary antibody: PACO18425(SPAG1 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes.
	The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO18425(SPAG1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.

Synonyms:

Sperm associated antigen 1

UniProt Protein Function:	SPAG1: Plays a role in fertilization. Binds GTP and has GTPase activity.
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA); Microtubule-binding Chromosomal Location of Human Ortholog: 8q22.2 Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm Molecular Function:GTP binding; hydrolase activity Biological Process: metabolic process; single fertilization Disease: Ciliary Dyskinesia, Primary, 28
UniProt Code:	Q07617
NCBI GenInfo Identifier:	223634718
NCBI Gene ID:
NCBI Accession:	Q07617.3
Molecular Weight:	104kDa
NCBI Full Name:	Sperm-associated antigen 1
UniProt Protein Name:	Sperm-associated antigen 1
UniProt Synonym Protein Names:	HSD-3.8; Infertility-related sperm protein Spag-1
Protein Family:	Sperm-associated antigen
UniProt Gene Name:	SPAG1
UniProt Entry Name:	SPAG1_HUMAN