Antibody Name:	Socs3 Antibody (PACO30064)
Antibody SKU:	PACO30064
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:2000
Species Reactivity:	Rat, Human
Immunogen:	Recombinant Rat Suppressor of cytokine signaling 3 protein (1-225AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western blot
All lanes: Socs3 antibody at 12µg/ml
Lane 1: K562 whole cell lysate
Lane 2: A375 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
.

Background:

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST (By similarity).

Synonyms:

Suppressor of cytokine signaling 3 (SOCS-3) (Cytokine-inducible SH2 protein 3), Socs3, Cish3

UniProt Protein Function:	SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
NCBI Summary:	regulates cytokine levels to modulate inflammation [RGD, Feb 2006]
UniProt Code:	O88583
NCBI GenInfo Identifier:	16758336
NCBI Gene ID:	89829
NCBI Accession:	NP_446017.1
UniProt Secondary Accession:	O88583,Q9QYV5,
UniProt Related Accession:	O88583
Molecular Weight:	24,808 Da
NCBI Full Name:	suppressor of cytokine signaling 3
NCBI Synonym Full Names:	suppressor of cytokine signaling 3
NCBI Official Symbol:	Socs3
NCBI Official Synonym Symbols:	Cish3; Ssi-3; Socs-3
NCBI Protein Information:	suppressor of cytokine signaling 3
UniProt Protein Name:	Suppressor of cytokine signaling 3
UniProt Synonym Protein Names:	Cytokine-inducible SH2 protein 3
Protein Family:	Suppressor of cytokine signaling
UniProt Gene Name:	Socs3
UniProt Entry Name:	SOCS3_RAT