Antibody Name:	SNX27 Antibody (PACO50854)
Antibody SKU:	PACO50854
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Sorting nexin-27 protein (458-497AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate. All lanes: SNX27 antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62, 60, 51 kDa. Observed band size: 51 kDa..
	Immunohistochemistry of paraffin-embedded human small intestine tissue using PACO50854 at dilution of 1:100.

Background:

Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.

Synonyms:

Sorting nexin-27, SNX27, KIAA0488

UniProt Protein Function:	SNX27: Involved in endocytic trafficking. In T lymphocytes, participates in endocytic recycling pathway. Recruits PSCDBP and HT4R to early endosomes. Belongs to the sorting nexin family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: cytoplasm; early endosome; immunological synapse; nucleoplasm; retromer complex Molecular Function:phosphatidylinositol 3-phosphate binding; protein binding Biological Process: endosome transport; establishment of natural killer cell polarity; intracellular protein transport
NCBI Summary:	This gene encodes a member of the sorting nexin family, a diverse group of cytoplasmic and membrane-associated proteins involved in endocytosis of plasma membrane receptors and protein trafficking through these compartments. All members of this protein family contain a phosphoinositide binding domain (PX domain). A highly similar protein in mouse is responsible for the specific recruitment of an isoform of serotonin 5-hydroxytryptamine 4 receptor into early endosomes, suggesting the analogous role for the human protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96L92
NCBI GenInfo Identifier:	166214988
NCBI Gene ID:	81609
NCBI Accession:	Q96L92.2
UniProt Secondary Accession:	Q96L92,Q32Q36, Q4AEJ5, Q5VWB0, Q5VWB1, Q5VWB2, Q6IPP6 Q86UB1, Q96D79, Q9H3K8,
UniProt Related Accession:	Q96L92
Molecular Weight:	27,781 Da
NCBI Full Name:	Sorting nexin-27
NCBI Synonym Full Names:	sorting nexin family member 27
NCBI Official Symbol:	SNX27
NCBI Official Synonym Symbols:	MRT1; MY014
NCBI Protein Information:	sorting nexin-27
UniProt Protein Name:	Sorting nexin-27
Protein Family:	Sorting nexin
UniProt Gene Name:	SNX27
UniProt Entry Name:	SNX27_HUMAN