SNX12 Antibody (PACO58600)
- SKU:
- PACO58600
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Applications:
- IP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SNX12 Antibody (PACO58600) |
| Antibody SKU: | PACO58600 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF, IP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500, IP:1:200-1:2000, |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Sorting nexin-12 protein (2-172AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO58600 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate. All lanes: SNX12 antibody at 2.83µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 20, 19 kDa. Observed band size: 20 kDa. |
 | Immunofluorescence staining of A549 cells with PACO58600 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | May be involved in several stages of intracellular trafficking. |
| Synonyms: | Sorting nexin-12, SNX12 |
| UniProt Protein Function: | May be involved in several stages of intracellular trafficking. |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members. A similar protein in mouse may be involved in regulating the neurite outgrowth. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| UniProt Code: | Q9UMY4 |
| NCBI GenInfo Identifier: | 50403807 |
| NCBI Gene ID: | 29934 |
| NCBI Accession: | Q9UMY4.3 |
| UniProt Secondary Accession: | Q9UMY4,Q8WUG9, F8W8K5 |
| UniProt Related Accession: | Q9UMY4 |
| Molecular Weight: | 18,342 Da |
| NCBI Full Name: | Sorting nexin-12 |
| NCBI Synonym Full Names: | sorting nexin 12 |
| NCBI Official Symbol: | SNX12 |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | sorting nexin-12 |
| UniProt Protein Name: | Sorting nexin-12 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Sorting nexin |
| UniProt Gene Name: | SNX12 |
| UniProt Entry Name: | SNX12_HUMAN |
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