Name: SMC6 Antibody (PACO61654)
SKU: PACO61654
Availability: OutOfStock

Antibody Name:	SMC6 Antibody (PACO61654)
Antibody SKU:	PACO61654
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Structural maintenance of chromosomes protein 6 protein (309-450AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO61654 diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome.

Synonyms:

Structural maintenance of chromosomes protein 6, SMC protein 6, SMC-6, hSMC6, SMC6, SMC6L1

UniProt Protein Function:	SMC6L1: Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Belongs to the SMC family. SMC6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA repair, damageChromosomal Location of Human Ortholog: 2p24.2Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; nucleolus; intracellular; nucleusMolecular Function: protein binding; ATP bindingBiological Process: telomere maintenance via recombination; double-strand break repair via homologous recombination
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q96SB8
NCBI GenInfo Identifier:	122070455
NCBI Gene ID:	79677
NCBI Accession:	Q96SB8.2
UniProt Secondary Accession:	Q96SB8
UniProt Related Accession:	Q96SB8
Molecular Weight:	126kDa
NCBI Full Name:	Structural maintenance of chromosomes protein 6
NCBI Synonym Full Names:	structural maintenance of chromosomes 6
NCBI Official Symbol:	SMC6
NCBI Official Synonym Symbols:	SMC-6; hSMC6; SMC6L1
NCBI Protein Information:	structural maintenance of chromosomes protein 6
UniProt Protein Name:	Structural maintenance of chromosomes protein 6
UniProt Synonym Protein Names:
Protein Family:	Structural maintenance of chromosomes protein
UniProt Gene Name:	SMC6
UniProt Entry Name:	SMC6_HUMAN