SLC22A25 Antibody (PACO55962)
- SKU:
- PACO55962
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SLC22A25 Antibody (PACO55962) |
| Antibody SKU: | PACO55962 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:100-1:300, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Solute carrier family 22 member 25 protein (31-145AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate. All lanes: SLC22A25 antibody at 6.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 kDa. Observed band size: 62 kDa. |
 | Immunofluorescence staining of HepG2 cells with PACO55962 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO55962 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | integral component of plasma membrane, inorganic anion exchanger activity, sodium-independent organic anion transmembrane transporter activity, urate transmembrane transporter activity, sodium-independent organic anion transport, urate transport |
| Synonyms: | Solute carrier family 22 member 25 (Organic anion transporter UST6), SLC22A25, UST6 |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q6T423 |
| NCBI GenInfo Identifier: | 153791484 |
| NCBI Gene ID: | 387601 |
| NCBI Accession: | NP_955384.3 |
| UniProt Secondary Accession: | Q6T423,A4FU27 |
| UniProt Related Accession: | Q6T423 |
| Molecular Weight: | 61,008 Da |
| NCBI Full Name: | solute carrier family 22 member 25 |
| NCBI Synonym Full Names: | solute carrier family 22 member 25 |
| NCBI Official Symbol: | SLC22A25 |
| NCBI Official Synonym Symbols: | UST6; HIMTP |
| NCBI Protein Information: | solute carrier family 22 member 25 |
| UniProt Protein Name: | Solute carrier family 22 member 25 |
| UniProt Synonym Protein Names: | Organic anion transporter UST6 |
| Protein Family: | Solute carrier family |
| UniProt Gene Name: | SLC22A25 |
| UniProt Entry Name: |
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