Antibody Name:	SLC22A17 Antibody (PACO38366)
Antibody SKU:	PACO38366
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Solute carrier family 22 member 17 protein (1-99AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: U87 whole cell lysate. All lanes: SLC22A17 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 56, 22 kDa. Observed band size: 58 kDa.
	Immunofluorescence staining of A549 cells with PACO38366 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO38366 diluted at 1:200 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis.

Synonyms:

Solute carrier family 22 member 17 (24p3 receptor) (24p3R) (Brain-type organic cation transporter) (Lipocalin-2 receptor) (Neutrophil gelatinase-associated lipocalin receptor) (NgalR), SLC22A17, BOCT BOIT

UniProt Protein Function:	SLC22A17: Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: integral to plasma membrane; vacuolar membrane Molecular Function:transmembrane receptor activity; transmembrane transporter activity Biological Process: cellular iron ion homeostasis; siderophore transport; ion transport; signal transduction; transmembrane transport
UniProt Code:	Q8WUG5
NCBI GenInfo Identifier:	27805426
NCBI Gene ID:	51310
NCBI Accession:	Q8WUG5.1
UniProt Related Accession:	Q8WUG5
Molecular Weight:
NCBI Full Name:	Solute carrier family 22 member 17
NCBI Synonym Full Names:	solute carrier family 22 member 17
NCBI Official Symbol:	SLC22A17
NCBI Official Synonym Symbols:	BOCT; BOIT; 24p3R; NGALR; hBOIT; NGALR2; NGALR3
NCBI Protein Information:	solute carrier family 22 member 17
UniProt Protein Name:	Solute carrier family 22 member 17
UniProt Synonym Protein Names:	24p3 receptor; 24p3R; Brain-type organic cation transporter; Lipocalin-2 receptor; Neutrophil gelatinase-associated lipocalin receptor; NgalR
UniProt Gene Name:	SLC22A17
UniProt Entry Name:	S22AH_HUMAN