Antibody Name:	SLC11A1 Antibody (PACO55778)
Antibody SKU:	PACO55778
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:100-1:300, IF:1:50-1:200
Species Reactivity:	Human, Rat
Immunogen:	Recombinant Human Natural resistance-associated macrophage protein 1 protein (1-58AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Rat heart tissue. All lanes: SLC11A1 antibody at 5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 48 kDa. Observed band size: 60 kDa.
	Immunofluorescence staining of HepG2 cells with PACO55778 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO55778 diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.

Synonyms:

Natural resistance-associated macrophage protein 1 (NRAMP 1) (Solute carrier family 11 member 1), SLC11A1, LSH NRAMP NRAMP1

UniProt Protein Function:	SLC11A1: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. Belongs to the NRAMP family.Protein type: Cell surface; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Vesicle; TransporterChromosomal Location of Human Ortholog: 2q35Cellular Component: late endosome; late endosome membrane; lysosome; phagocytic vesicle membrane; plasma membraneMolecular Function: manganese ion transmembrane transporter activity; metal ion:hydrogen antiporter activity; protein homodimerization activity; transition metal ion transmembrane transporter activityBiological Process: activation of protein kinase activity; antigen processing and presentation of peptide antigen; cell redox homeostasis; cellular cadmium ion homeostasis; cellular iron ion homeostasis; defense response to bacterium; defense response to Gram-negative bacterium; defense response to protozoan; inflammatory response; interleukin-2 production; interleukin-3 production; iron ion homeostasis; iron ion transport; L-arginine import; macrophage activation; manganese ion transport; MHC class II biosynthetic process; mRNA stabilization; negative regulation of cytokine production; nitrite transport; phagocytosis; positive regulation of cytokine production; positive regulation of dendritic cell antigen processing and presentation; positive regulation of interferon-gamma production; positive regulation of phagocytosis; positive regulation of T-helper 1 type immune response; positive regulation of transcription from RNA polymerase II promoter; respiratory burst; response to bacterium; response to lipopolysaccharide; T cell cytokine production; T cell proliferation during immune response; vacuolar acidification; wound healingDisease: Buruli Ulcer, Susceptibility To; Mycobacterium Tuberculosis, Susceptibility To
UniProt Protein Details:
NCBI Summary:	This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	P49279
NCBI GenInfo Identifier:	1352521
NCBI Gene ID:	6556
NCBI Accession:	P49279.1
UniProt Secondary Accession:	P49279,C0H5Y3
UniProt Related Accession:	P49279
Molecular Weight:	47,292 Da
NCBI Full Name:	Natural resistance-associated macrophage protein 1
NCBI Synonym Full Names:	solute carrier family 11 member 1
NCBI Official Symbol:	SLC11A1
NCBI Official Synonym Symbols:	LSH; NRAMP; NRAMP1
NCBI Protein Information:	natural resistance-associated macrophage protein 1
UniProt Protein Name:	Natural resistance-associated macrophage protein 1
UniProt Synonym Protein Names:	Solute carrier family 11 member 1
Protein Family:	Natural resistance-associated macrophage protein
UniProt Gene Name:	SLC11A1
UniProt Entry Name:	NRAM1_HUMAN