Antibody Name:	SKA3 Antibody (PACO57316)
Antibody SKU:	PACO57316
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Spindle and kinetochore-associated protein 3 protein (101-195AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	IHC image of PACO57316 diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	Immunofluorescence staining of Hela cells with PACO57316 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO57316 diluted at 1:300 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules.

Synonyms:

Spindle and kinetochore-associated protein 3, SKA3, C13orf3 RAMA1

UniProt Protein Function:	RAMA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Belongs to the SKA3 family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 13q12.11Cellular Component: outer kinetochore of condensed chromosome; spindle microtubuleMolecular Function: protein bindingBiological Process: chromosome segregation; mitosis; regulation of microtubule polymerization or depolymerization
UniProt Protein Details:
NCBI Summary:	This gene encodes a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. The encoded protein localizes to the outer kinetechore and may be required for normal chromosome segregation and cell division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Code:	Q8IX90
NCBI GenInfo Identifier:	73620123
NCBI Gene ID:	221150
NCBI Accession:	Q8IX90.2
UniProt Secondary Accession:	Q8IX90,Q5VZV5, Q86WR2, Q8NBG1, Q96D22, A2A330, A2A331 B2RBY2
UniProt Related Accession:	Q8IX90
Molecular Weight:	43,978 Da
NCBI Full Name:	Spindle and kinetochore-associated protein 3
NCBI Synonym Full Names:	spindle and kinetochore associated complex subunit 3
NCBI Official Symbol:	SKA3
NCBI Official Synonym Symbols:	RAMA1; C13orf3
NCBI Protein Information:	spindle and kinetochore-associated protein 3
UniProt Protein Name:	Spindle and kinetochore-associated protein 3
UniProt Synonym Protein Names:
Protein Family:	Spindle and kinetochore-associated protein
UniProt Gene Name:	SKA3
UniProt Entry Name:	SKA3_HUMAN