Name: SKA2 Antibody (PACO59253)
SKU: PACO59253
Availability: OutOfStock

Antibody Name:	SKA2 Antibody (PACO59253)
Antibody SKU:	PACO59253
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Spindle and kinetochore-associated protein 2 protein (1-121AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO59253 diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of PACO59253 diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules.

Synonyms:

Spindle and kinetochore-associated protein 2 (Protein FAM33A), SKA2, FAM33A

UniProt Protein Function:	Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:17093495, PubMed:19289083). In the complex, it is required for SKA1 localization (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q8WVK7
NCBI GenInfo Identifier:	74762633
NCBI Gene ID:	348235
NCBI Accession:	Q8WVK7.1
UniProt Secondary Accession:	Q8WVK7,A6NIL3, B3KPL3, E9PCB8
UniProt Related Accession:	Q8WVK7
Molecular Weight:	8,282 Da
NCBI Full Name:	Spindle and kinetochore-associated protein 2
NCBI Synonym Full Names:	spindle and kinetochore associated complex subunit 2
NCBI Official Symbol:	SKA2
NCBI Official Synonym Symbols:	FAM33A
NCBI Protein Information:	spindle and kinetochore-associated protein 2
UniProt Protein Name:	Spindle and kinetochore-associated protein 2
UniProt Synonym Protein Names:	Protein FAM33A
Protein Family:	Spindle and kinetochore-associated protein
UniProt Gene Name:	SKA2
UniProt Entry Name:	SKA2_HUMAN