Name: SIRPB1 Antibody (PACO59133)
SKU: PACO59133
Availability: OutOfStock

Antibody Name:	SIRPB1 Antibody (PACO59133)
Antibody SKU:	PACO59133
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Signal-regulatory protein beta-1 isoform 3 protein (30-206AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	IHC image of PACO59133 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	Immunofluorescence staining of MCF-7 cells with PACO59133 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO59133 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes.
Synonyms:	Signal-regulatory protein beta-1 isoform 3 (SIRP-beta-1 isoform 3), SIRPB1

UniProt Protein Function:	SIRPB1 iso3: Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 20p13Cellular Component: integral to plasma membrane; plasma membraneMolecular Function: protein bindingBiological Process: cell surface receptor linked signal transduction; innate immune response; signal transduction
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
UniProt Code:	Q5TFQ8
NCBI GenInfo Identifier:	205829937
NCBI Gene ID:	10326
NCBI Accession:	Q5TFQ8.1
UniProt Secondary Accession:	Q5TFQ8,Q7Z3B9
UniProt Related Accession:	Q5TFQ8, O00241
Molecular Weight:	43,211 Da
NCBI Full Name:	Signal-regulatory protein beta-1 isoform 3
NCBI Synonym Full Names:	signal regulatory protein beta 1
NCBI Official Symbol:	SIRPB1
NCBI Official Synonym Symbols:	CD172b; SIRP-BETA-1
NCBI Protein Information:	signal-regulatory protein beta-1
UniProt Protein Name:	Signal-regulatory protein beta-1 isoform 3
UniProt Synonym Protein Names:
Protein Family:	Signal-regulatory protein
UniProt Gene Name:	SIRPB1
UniProt Entry Name:	SIRBL_HUMAN