Sialic Acid Assay Kit (Colorimetric or Fluorometric) (BA0058)

Sialic Acid Assay Kit - Information

Assay Genie's sialic acid assay uses an improved Warren method, in which sialic acid is oxidized to formylpyruvic acid which reacts with thiobarbituric acid to form a pink colored product. The color intensity at 549 nm or fluorescence intensity at Ex/Em = 555/585 nm is directly proportional to sialic acid concentration in the sample.

Applications

For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on sialic acid.

Sialic Acid Assay Kit - Key Features

• Sensitive and accurate. Use as little as 60 samples. Linear detection range in 96-well plate: 5 to 1000 sialic acid for colorimetric assays and 0.5 to 100 for fluorimetric assays.

Sialic Acid Assay Kit - Data Sheet
Kit Includes	Dye Reagent: 6 mL Oxidation Reagent: 10 mL 10% TCA: 5 mL Hydrolysis Reagent: 10 mL DMSO: 12 mL Standard: 500 mL 10 mM Sialic Acid
Kit Requires	Pipeting devices, centrifuge tubes, centrifuge, heat block, clear flat-bottom 96-well plates, black 96-well plates and plate readers
Method of Detection	OD549nm, or FL555/585nm
Detection Limit	OD, FL: 5, 0.5
Samples	Serum, plasma, saliva, milk etc
Species	All
Protocol Length	2.5 hrs
Size	100 tests
Storage	Store the Standard at -20°C, all others at room temperature
Shelf Life	12 months

More Details

SIALIC ACID is a general name for nine carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple and direct procedures for measuring sialic acid concentrations find wide applications in research and drug discovery.