Sialic Acid 1-Step Assay Kit (BA0148)

About Sialic Acid:

Sialic acid is a general name for nine carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The Sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple, direct and automation-ready procedures for measuring sialic acid concentrations find wide applications in research and drug discovery.

How our Sialic Acid Assay Kit Works:

Assay Genie's sialic acid assay uses a single Working Reagent that combines NANA aldolase, pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to sialic acid concentration in the sample

Key Features:

• Sensitive and accurate. Use as little as 10 uL samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 uM for fluorimetric assays.
• Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step.

Sialic Acid Assay Kit Information:

Application:	For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism.
Kit Includes:	Assay Buffer: 10 mL Hydrolysis Reagent: 10 mL Enzyme: 120 uL Neutraliztion Reagent: 5 mL Dye Reagent: 120 uL Standard: 500 uL 10 mM Sialic Acid
Kit Requires:	Pipeting devices, centrifuge tubes, Clear flat-bottom 96-well plates, black 96-well or 384-well plates and plate reader.
Method of Detection:	OD570nm, or FL530/585nm
Detection Limit:	OD, FL: 20, 2 uM
Samples:	Biological
Species:	All
Protocol Length:	2 hrs
Size:	100 tests
Storage:	Store all kit components at -20°C.
Shelf Life:	6 months