Name: SHROOM2 Antibody (PACO57152)
SKU: PACO57152
Availability: OutOfStock

Antibody Name:	SHROOM2 Antibody (PACO57152)
Antibody SKU:	PACO57152
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Protein Shroom2 protein (213-405AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Immunofluorescence staining of MCF-7 cells with PACO57152 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of PACO57152 diluted at 1:200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution (By similarity).
Synonyms:	Protein Shroom2 (Apical-like protein) (Protein APXL), SHROOM2, APXL

UniProt Protein Function:	APXL: May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution. Belongs to the shroom family.Protein type: Channel, sodiumChromosomal Location of Human Ortholog: Xp22.3Cellular Component: apical plasma membrane; cell-cell adherens junction; cortical actin cytoskeleton; cytoskeleton; plasma membrane; tight junctionMolecular Function: actin binding; actin filament binding; amiloride-sensitive sodium channel activity; beta-catenin binding; protein bindingBiological Process: actin filament bundle formation; apical protein localization; brain development; camera-type eye development; camera-type eye morphogenesis; cell migration; cell morphogenesis; cellular pigment accumulation; ear development; establishment of melanosome localization; eye pigment granule organization and biogenesis; lens morphogenesis in camera-type eye; melanosome organization and biogenesis
UniProt Protein Details:
NCBI Summary:	This gene represents the human homolog of Xenopus laevis apical protein (APX) gene, which is implicated in amiloride-sensitive sodium channel activity. It is expressed in endothelial cells and facilitates the formation of a contractile network within endothelial cells. Depletion of this gene results in an increase in endothelial sprouting, migration, and angiogenesis. This gene is highly expressed in the retina, and is a strong candidate for ocular albinism type 1 syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]
UniProt Code:	Q13796
NCBI GenInfo Identifier:	2498147
NCBI Gene ID:	357
NCBI Accession:	Q13796.1
UniProt Secondary Accession:	Q13796,B9EIQ7
UniProt Related Accession:	Q13796
Molecular Weight:	176,410 Da
NCBI Full Name:	Protein Shroom2
NCBI Synonym Full Names:	shroom family member 2
NCBI Official Symbol:	SHROOM2
NCBI Official Synonym Symbols:	APXL; HSAPXL
NCBI Protein Information:	protein Shroom2
UniProt Protein Name:	Protein Shroom2
UniProt Synonym Protein Names:	Apical-like protein; Protein APXL
Protein Family:	Protein
UniProt Gene Name:	SHROOM2
UniProt Entry Name:	SHRM2_HUMAN