SHPRH Antibody (PACO58144)
- SKU:
- PACO58144
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SHPRH Antibody (PACO58144) |
| Antibody SKU: | PACO58144 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human E3 ubiquitin-protein ligase SHPRH protein (1362-1512AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO58144 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of SH-SY5Y cells with PACO58144 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. |
| Synonyms: | E3 ubiquitin-protein ligase SHPRH (EC 2.3.2.27) (EC 3.6.4) (RING-type E3 ubiquitin transferase SHPRH) (SNF2, histone-linker, PHD and RING finger domain-containing helicase), SHPRH, KIAA2023 |
| UniProt Protein Function: | SHPRH: E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. Belongs to the SNF2/RAD54 helicase family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.6.1.-; EC 3.6.4.-; EC 6.3.2.-; EC 6.3.2.19; Helicase; Ligase; Ubiquitin conjugating system; Ubiquitin ligaseChromosomal Location of Human Ortholog: 6q24.3Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activityBiological Process: protein polyubiquitination; response to DNA damage stimulus |
| UniProt Protein Details: | |
| NCBI Summary: | SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q149N8 |
| NCBI GenInfo Identifier: | 109734409 |
| NCBI Gene ID: | 257218 |
| NCBI Accession: | BC117686 |
| UniProt Secondary Accession: | Q149N8,Q149N9, Q5VV79, Q68DS5, Q7Z5J5, Q8IVE8, Q8IWQ9 Q8N1S8, Q8NBR7 |
| UniProt Related Accession: | Q149N8 |
| Molecular Weight: | 54,339 Da |
| NCBI Full Name: | Homo sapiens SNF2 histone linker PHD RING helicase, mRNA |
| NCBI Synonym Full Names: | SNF2 histone linker PHD RING helicase |
| NCBI Official Symbol: | SHPRH |
| NCBI Official Synonym Symbols: | bA545I5.2 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase SHPRH |
| UniProt Protein Name: | E3 ubiquitin-protein ligase SHPRH |
| UniProt Synonym Protein Names: | SNF2, histone-linker, PHD and RING finger domain-containing helicase |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | SHPRH |
| UniProt Entry Name: | SHPRH_HUMAN |
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