Hormone & Small Molecule ELISA Kits
Serotonin ELISA Kit
- SKU:
- UNFI0018
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Sensitivity:
- 0.938ng/ml
- Range:
- 1.563-100ng/ml
- ELISA Type:
- Competitive
- Synonyms:
- ST, 5-HT, Serotonin, 5-Hydroxytryptamine
- Reactivity:
- Universal
Description
Serotonin ELISA Kit
Serotonin is a neurotransmitter and hormone that plays a crucial role in regulating mood, sleep, appetite, and various other physiological processes in the body. It is involved in numerous biological functions and is often implicated in psychiatric and neurological disorders. The Serotonin ELISA Kit is specifically designed to measure serotonin levels in biological samples, allowing researchers to accurately quantify and analyze serotonin concentrations, facilitating a better understanding of its functions and associated conditions.
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | ST/5-HT (5-hydroxytryptamine) ELISA Kit |
| Product Code: | UNFI0018 |
| Size: | 96 Assays |
| Alias: | ST, 5-HT, Serotonin, 5-Hydroxytryptamine |
| Detection Method: | Competitive ELISA, Coated with Antibody |
| Reactivity: | Universal |
| Sensitivity: | 0.938ng/ml |
| Range: | 1.563-100ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of ST/5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of ST/5-HT in samples.
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ST/5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) | Intra Assay <8 Inter Assay <10 |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 2-8°C/-20°C |
| Lyophilized Standard | 2 | 2-8°C/-20°C |
| Sample/Standard Dlution Buffer | 20ml | 2-8°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 2-8°C (Avoid Direct Light) |
| Antibody Dilution Buffer | 10ml | 2-8°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 2-8°C (Avoid Direct Light) |
| SABC Dilution Buffer | 10ml | 2-8°C |
| TMB Substrate | 10ml | 2-8°C (Avoid Direct Light) |
| Stop Solution | 10ml | 2-8°C |
| Wash Buffer (25X) | 30ml | 2-8°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
Protocol
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Equilibrate the TMB substrate for at least 30 min at 37°C beforeuse. When diluting samples and reagents, they must be mixed completely andevenly. It is recommended to plot a standard curve for each test.
| Step | Procedure |
| 1. | Set standard, test sample and control (zero) wells on the pre-coatedplate respectively, and then, record their positions. It isrecommended to measure each standard and sample in duplicate. Washplate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Add Sample and Biotin-detection antibody: Add 50µL of Standard, Blank or Sample per well. The blankwell is added with Sample Dilution Buffer. Immediately add 50 µL of biotin-labelled antibody workingsolution to each well. Cover with the plate sealer provided. Gently tap the plate to ensure thoroughmixing. Incubate for 45 minutes at 37°C. (Solutions are added to the bottom of micro-ELISA platewell, avoid touching plate walls and foaming). |
| 3. | Wash: Aspirate each well and wash, repeating the process three timesWash by filling each well with Wash Buffer (approximately 350µL)using a squirt bottle, multi-channel pipette, manifold dispenser orautomated washer. Complete removal of liquid at each step is essentialto good performance. After the last wash, remove any remaining WashBuffer by aspirating or decanting. Invert the plate and pat it againstthick clean absorbent paper. |
| 4. | HRP-Streptavidin Conjugate(SABC): Add 100µL of SABC workingsolution to each well. Cover with a new Plate sealer. Incubate for30minutes at 37°C. |
| 5. | Wash: Repeat the aspiration/wash process for five times. |
| 6. | TMB Substrate: Add 90µL of TMB Substrate to each well. Coverwith a new Plate sealer. Incubate for about 10-20 minutes at 37°C.Protect from light. The reaction time can be shortened or extendedaccording to the actual color change, but not more than 30minutes.When apparent gradient appeared in standard wells, you can terminatethe reaction. |
| 7. | Stop: Add 50µL of Stop Solution to each well. Color turn toyellow immediately. The adding order of stop solution should be as thesame as the substrate solution. |
| 8. |
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