Senescence Detection Kit (SA-beta-gal Staining) (CV0018)

Kit Size:

100 Assays

Features & Benefits:

• Fast, convenient and easy procedure: takes 28 minutes to results with minimal handling steps.
• The specific histochemical marker is only present in senescent cells and is not found in pre-senescent, quiescent or immortal cells

Kit Contents:

• 150 mg X-Gal (lyophilized)
• 60 mL PBS (10x)
• 15 mL Fixative Solution (10x)
• 15 mL Staining Solution (10x)
• 1 x 1,5 mL Staining Solution Supplement A (100x)
• 1 x 1,5 mL Staining Solution Supplement B (100x)

Applications:

Histochemically detect SA-beta-Galactosidase activity in cultured cell and tissue sections.

Kit Advice:

• The fixative solution contains formaldehyde and glutaraldehyde, which are toxic and corrosive solutions. Wear personal protective clothing when handling solutions and use in a fume hood.
• Staining solution supplements (A and B) contains K4[Fe(CN)6] 3H2O and K3[Fe(CN)6], which are irritants for humans and dangerous for the environment. Wear personal protective clothing (e.g., nitrile or latex gloves, lab coat and goggles) when handling solution and discard in an appropriate manner.
• Dimethylformamide is toxic and harmful. Wear personal protective clothing when handling solution. Use a fume hood. Always use a polypropylene container or glass to make and store the X-gal. Do not use polystyrene.

Shipping & Storage:

• Shipped in: Gel Pack.
• Storage: 4 ºC.
• Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety:

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.