SEMA4B Antibody (PACO56170)
- SKU:
- PACO56170
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SEMA4B Antibody (PACO56170) |
| Antibody SKU: | PACO56170 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:100-1:300, IF:1:50-1:200 |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant Human Semaphorin-4B protein (163-253AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Rat luing tissue. All lanes: SEMA4B antibody at 6.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 93, 82 kDa. Observed band size: 93 kDa. |
 | IHC image of PACO56170 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO56170 diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. |
| Synonyms: | Semaphorin-4B, SEMA4B, KIAA1745 SEMAC |
| UniProt Protein Function: | SEMA4B iso3: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Two alternatively spliced human isoforms have been described.Protein type: Cell surface; Membrane protein, integralChromosomal Location of Human Ortholog: 15q25Cellular Component: integral to membrane; plasma membrane; synapseMolecular Function: receptor activityBiological Process: nervous system development; cell differentiation |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9NPR2 |
| NCBI GenInfo Identifier: | 29840873 |
| NCBI Gene ID: | 10509 |
| NCBI Accession: | Q9NPR2.3 |
| UniProt Secondary Accession: | Q9NPR2,Q6UXE3, Q8WVP9, Q96FK5, Q9C0B8, Q9H691, Q9NPM8 Q9NPN0 |
| UniProt Related Accession: | Q9NPR2 |
| Molecular Weight: | 81,403 Da |
| NCBI Full Name: | Semaphorin-4B |
| NCBI Synonym Full Names: | sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B |
| NCBI Official Symbol: | SEMA4B |
| NCBI Official Synonym Symbols: | SemC; SEMAC |
| NCBI Protein Information: | semaphorin-4B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4B |
| UniProt Protein Name: | Semaphorin-4B |
| UniProt Synonym Protein Names: | |
| Protein Family: | Semaphorin |
| UniProt Gene Name: | SEMA4B |
| UniProt Entry Name: | SEM4B_HUMAN |
| Antibodies |
| SEMA4B Antibody (PACO12094) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
Related Products
Human SEMA4B Recombinant Protein (RPES4281)
system_update_altDatasheetHuman SEMA4B Recombinant Protein Semaphorin-4B is a single-pass type I membrane protein. SEMA4B is a member of the semaphorin family. The class 4 semaphorins are integra
Human SEMA4B Recombinant Protein (RPES4256)
system_update_altDatasheetHuman SEMA4B Recombinant Protein The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. SEMA4B is a single-pass
SIM1 Antibody
Overview Product Name:SIM1 AntibodyProduct Code:PACO64949Size:50µLTarget Names:SIM1Species Reactivity:HumanHost