sdeA Antibody (PACO63047)
- SKU:
- PACO63047
- Product Type:
- Antibody
- Reactivity:
- Bacteria
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | sdeA Antibody (PACO63047) |
| Antibody SKU: | PACO63047 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
| Species Reactivity: | Legionella pneumophila subsp |
| Immunogen: | Recombinant Legionella pneumophila subsp Ubiquitinating/deubiquitinating enzyme SdeA protein (19-268AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in Recombinant protein. All lanes: sdeA antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa. |
| Background: | Secreted effector that interferes with the host cell ubiquitin pathway and is required for intracellular bacterial replication. Catalyzes the ubiquitination of several mammalian Rab proteins (Rab33b, Rab1, Rab6a and Rab30) during L.pneumophila infection, without engaging the standard cellular enzyme cascade (E1 and E2). Transfers an ADP-ribose moiety from NAD to the 'Arg-42' residue of ubiquitin in a reaction that releases nicotinamide. The modified ubiquitin is subsequently transferred to the substrate protein through an unknown mechanism that results in the release of AMP. Cannot ubiquitinate the endosomal Rab5 or the cytoskeletal small GTPase Rac1. Also acts as a deubiquitinase (DUB), catalyzing the cleavage of three of the most abundant polyubiquitin chains ('Lys-11', 'Lys-48' and 'Lys-63') with a distinct preference for 'Lys-63' linkages; is thus able to efficiently remove 'Lys-63'-linked polyubiquitin chains from the phagosomal surface. Is also able to remove NEDD8 from neddylated proteins, but is unable to recognize SUMO. The DUB activity of SdeA is important for regulating the dynamics of ubiquitin association with the bacterial phagosome, but is dispensable for its role in intracellular bacterial replication. |
| Synonyms: | Ubiquitinating/deubiquitinating enzyme SdeA (Effector protein SdeA) [Includes: Deubiquitinase (DUB) (EC 3.4.22) (Deneddylase) (Deubiquitinating enzyme); Ubiquitin transferase (EC 2.3.2); Mono-ADP-ribosyltransferase (mART) (EC 2.4.2.31)], sdeA |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q5ZTK4 |
| NCBI GenInfo Identifier: | 1064303039 |
| NCBI Gene ID: | |
| NCBI Accession: | |
| UniProt Secondary Accession: | Q5ZTK4 |
| UniProt Related Accession: | Q5ZTK4 |
| Molecular Weight: | |
| NCBI Full Name: | Ubiquitinating/deubiquitinating enzyme SdeA |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | |
| UniProt Protein Name: | |
| UniProt Synonym Protein Names: | |
| Protein Family: | Ubiquitinating/deubiquitinating enzyme |
| UniProt Gene Name: | |
| UniProt Entry Name: |
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