Name: SCARA5 Antibody (PACO55766)
SKU: PACO55766
Availability: OutOfStock

Antibody Name:	SCARA5 Antibody (PACO55766)
Antibody SKU:	PACO55766
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Scavenger receptor class A member 5 protein (303-428AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO55766 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with PACO55766 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1).

Synonyms:

Scavenger receptor class A member 5 (Scavenger receptor hlg), SCARA5

UniProt Protein Function:	SCARA5: Ferritin receptor that mediates non-transferrin- dependent delivery of iron. Mediates cellular uptake of ferritin- bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type- specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1). Belongs to the SCARA5 family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 8p21.1Cellular Component: cell surface; integral to plasma membraneMolecular Function: scavenger receptor activityBiological Process: receptor-mediated endocytosis; cellular iron ion homeostasis; regulation of gene expression; regulation of blood coagulation; endocytosis
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q6ZMJ2
NCBI GenInfo Identifier:	47271477
NCBI Gene ID:	286133
NCBI Accession:	NP_776194.2
UniProt Secondary Accession:	Q6ZMJ2,Q6UXZ1, Q7Z4A1, Q8N4Z7
UniProt Related Accession:	Q6ZMJ2
Molecular Weight:	28,871 Da
NCBI Full Name:	scavenger receptor class A member 5
NCBI Synonym Full Names:	scavenger receptor class A, member 5
NCBI Official Symbol:	SCARA5
NCBI Official Synonym Symbols:	Tesr; NET33
NCBI Protein Information:	scavenger receptor class A member 5; scavenger receptor hlg; testis expressed scavenger receptor; scavenger receptor class A, member 5 (putative)
UniProt Protein Name:	Scavenger receptor class A member 5
UniProt Synonym Protein Names:	Scavenger receptor hlg
Protein Family:
UniProt Gene Name:	SCARA5
UniProt Entry Name:	SCAR5_HUMAN