Description
Antibody Name: | SBK2 Antibody (PACO62371) |
Antibody SKU: | PACO62371 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:50-1:500, IF:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Serine/threonine-protein kinase SBK2 protein (62-330AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO62371 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO62371 diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of A549 cells with PACO62371 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | cytoplasm, protein serine/threonine kinase activity, Rac GTPase binding, actin cytoskeleton organization, activation of protein kinase activity, cell migration, regulation of apoptotic process, regulation of MAPK cascade, regulation of mitotic cell cycle, Rho protein signal transduction |
Synonyms: | Serine/threonine-protein kinase SBK2 (EC 2.7.11.1) (SH3-binding domain kinase family member 2) (Sugen kinase 69) (SgK069), SBK2, SGK069 |
UniProt Protein Function: | |
UniProt Protein Details: | |
NCBI Summary: | |
UniProt Code: | P0C263 |
NCBI GenInfo Identifier: | 301500720 |
NCBI Gene ID: | 646643 |
NCBI Accession: | NP_001094871.2 |
UniProt Secondary Accession: | P0C263 |
UniProt Related Accession: | P0C263 |
Molecular Weight: | 38,088 Da |
NCBI Full Name: | serine/threonine-protein kinase SBK2 |
NCBI Synonym Full Names: | SH3 domain binding kinase family member 2 |
NCBI Official Symbol: | SBK2 |
NCBI Official Synonym Symbols: | SGK069 |
NCBI Protein Information: | serine/threonine-protein kinase SBK2 |
UniProt Protein Name: | Serine/threonine-protein kinase SBK2 |
UniProt Synonym Protein Names: | SH3-binding domain kinase family member 2; Sugen kinase 69; SgK069 |
Protein Family: | Serine/threonine-protein kinase |
UniProt Gene Name: | SBK2 |
UniProt Entry Name: |