Name: RUNX3 Transcription Factor Activity Assay
SKU: TFAB00075
Availability: OutOfStock

Product Name:	RUNX3 Transcription Factor Activity Assay
Product Code:	TFAB00075
Target:	RUNX3
Synonyms:	Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3, CBF-alpha-3, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEA2-alpha C, PEBP2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit
Reactivity:	Human, Mouse, Rat
Sample Types:	Nuclear or cell lysates

The Assay Genie RUNX3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:	4.5 hours
Detection Method:	Colorimetric 450 nm
Size:	12 x 8-Well Microstrips
Storage:	4°C for 6 months

UniProt Protein Function:	AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 1p36 Cellular Component: cytoplasm; nuclear chromatin; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: chondrocyte differentiation; hemopoiesis; negative regulation of cell cycle; negative regulation of epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; ossification; peripheral nervous system neuron development; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cell differentiation; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Code:	Q13761
NCBI GenInfo Identifier:	17368453
NCBI Gene ID:	864
NCBI Accession:	Q13761.2
UniProt Secondary Accession:	Q13761,Q12969, Q13760, B1AJV5,
UniProt Related Accession:	Q13761
Molecular Weight:	45,922 Da
NCBI Full Name:	Runt-related transcription factor 3
NCBI Synonym Full Names:	runt related transcription factor 3
NCBI Official Symbol:	RUNX3
NCBI Official Synonym Symbols:	AML2; CBFA3; PEBP2aC
NCBI Protein Information:	runt-related transcription factor 3
UniProt Protein Name:	Runt-related transcription factor 3
UniProt Synonym Protein Names:	Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit
Protein Family:	Runt-related transcription factor
UniProt Gene Name:	RUNX3
UniProt Entry Name:	RUNX3_HUMAN