Description
| Product Name: | RNF8 Monoclonal Antibody |
| Product Code: | CAB22524 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western blotting, Immunohistochemistry |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Clone No: | ARC58373 |
| Reactivity: | Human, Mouse, Rat |
| Tested Applications: | WB, IHC-P, ELISA |
| Key Applications: | Western blotting, Immunohistochemistry |
| Recommended Dilution: | WB 1:1000-1:5000, IHC-P 1:100-1:500 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Positive Samples: | 293T, Jurkat, HeLa, Mouse brain, Rat brain |
| Cellular Location: | Chromosome, Midbody, Nucleus, telomere |
The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants.
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNF8 (NP_003949.1). |
| Sequence: | MGEPG FFVTG DRAGG RSWCL RRVGM SAGWL LLEDG CEVTV GRGFG VTYQL VSKIC PLMIS RNHCV LKQNP EGQWT IMDNK SLNGV WLNRA RLEPL RVYSI HQGDY IQLGV PLENK ENAEY EYEVT EEDWE TIYPC LSPKN DQMIE KNKEL RTKRK FSLDE LAGPG AEGPS NLKSK INKVS CESGQ PVKSQ GKGEV ASTPS DNLDP KLTAL EPSKT TGAPI YPGFP KVTEV HHEQK ASNSS ASQRS LQMFK VTMSR ILRLK |
| Synonyms: | hRNF8 |
| Calculated MW: | 56kDa |
| Observed MW: | 62kDa |
