RNF43 Antibody (PACO57948)
- SKU:
- PACO57948
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | RNF43 Antibody (PACO57948) |
| Antibody SKU: | PACO57948 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human E3 ubiquitin-protein ligase RNF43 protein (566-697AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO57948 diluted at 1:600 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. |
| Synonyms: | E3 ubiquitin-protein ligase RNF43 (EC 2.3.2.27) (RING finger protein 43) (RING-type E3 ubiquitin transferase RNF43), RNF43 |
| UniProt Protein Function: | RNF43: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. Belongs to the ZNRF3 family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Membrane protein, integralChromosomal Location of Human Ortholog: 17q22Cellular Component: integral to plasma membrane; plasma membraneMolecular Function: frizzled binding; protein binding; ubiquitin-protein ligase activityBiological Process: negative regulation of Wnt receptor signaling pathway; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015] |
| UniProt Code: | Q68DV7 |
| NCBI GenInfo Identifier: | 74757361 |
| NCBI Gene ID: | 54894 |
| NCBI Accession: | Q68DV7.1 |
| UniProt Secondary Accession: | Q68DV7,Q65ZA4, Q6AI04, Q9NXD0, A8K4R2, B7Z443, B7Z5D5 B7Z5J5 |
| UniProt Related Accession: | Q68DV7 |
| Molecular Weight: | 95,038 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase RNF43 |
| NCBI Synonym Full Names: | ring finger protein 43 |
| NCBI Official Symbol: | RNF43 |
| NCBI Official Synonym Symbols: | URCC; RNF124 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RNF43 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RNF43 |
| UniProt Synonym Protein Names: | RING finger protein 43 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | RNF43 |
| UniProt Entry Name: | RNF43_HUMAN |
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