RHBDD3 Antibody (PACO63403)
- SKU:
- PACO63403
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | RHBDD3 Antibody (PACO63403) |
| Antibody SKU: | PACO63403 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Peptide sequence from Human Rhomboid domain-containing protein 3 protein (304-323AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: RHBDD3 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa. Observed band size: 41 kDa. |
 | Immunofluorescence staining of U251 cells with PACO63403 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO63403 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Synonyms: | Rhomboid domain-containing protein 3, RHBDD3, C22orf3 |
| UniProt Protein Function: | RHBDD3: Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 22q12.2Cellular Component: integral to membraneMolecular Function: serine-type endopeptidase activityBiological Process: response to xenobiotic stimulus; regulation of protein secretion; regulation of acute inflammatory response; negative regulation of natural killer cell activation; positive regulation of protein catabolic process; MAPKKK cascade; liver development; proteolysis |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9Y3P4 |
| NCBI GenInfo Identifier: | 11072101 |
| NCBI Gene ID: | 25807 |
| NCBI Accession: | NP_036397.1 |
| UniProt Secondary Accession: | Q9Y3P4,Q6I9X3, Q9UGQ7 |
| UniProt Related Accession: | Q9Y3P4 |
| Molecular Weight: | 40,484 Da |
| NCBI Full Name: | rhomboid domain-containing protein 3 |
| NCBI Synonym Full Names: | rhomboid domain containing 3 |
| NCBI Official Symbol: | RHBDD3 |
| NCBI Official Synonym Symbols: | PTAG; C22orf3; HS984G1A |
| NCBI Protein Information: | rhomboid domain-containing protein 3; pituitary tumor apoptosis |
| UniProt Protein Name: | Rhomboid domain-containing protein 3 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Rhomboid domain-containing protein |
| UniProt Gene Name: | RHBDD3 |
| UniProt Entry Name: | RHBD3_HUMAN |
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