Name: Rgn Antibody (PACO29480)
SKU: PACO29480
Availability: OutOfStock

Antibody Name:	Rgn Antibody (PACO29480)
Antibody SKU:	PACO29480
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:2000
Species Reactivity:	Rat, Human
Immunogen:	Recombinant Rat Regucalcin protein (1-299AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western blot
All lanes: Rgn antibody at 8µg/ml
Lane 1: A549 whole cell lysate
Lane 2: HCT116 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
.

Background:	Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Catalyzes a key step in ascorbic acid, (vitamin C) biosynthesis. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities.
Synonyms:	Regucalcin (RC) (Gluconolactonase) (GNL) (EC 3.1.1.17) (Senescence marker protein 30) (SMP-30), Rgn, Smp30

UniProt Protein Function:	RGN: Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities. Belongs to the SMP-30/CGR1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.1.1.17 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:calcium ion binding; enzyme regulator activity; gluconolactonase activity; zinc ion binding Biological Process: aging; cellular calcium ion homeostasis; kidney development; L-ascorbic acid biosynthetic process; liver development; negative regulation of apoptosis; negative regulation of cyclic-nucleotide phosphodiesterase activity; negative regulation of epithelial cell proliferation; negative regulation of nitric oxide biosynthetic process; negative regulation of phosphoprotein phosphatase activity; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase activity; positive regulation of ATPase activity; positive regulation of fatty acid biosynthetic process; positive regulation of GTPase activity; regulation of calcium-mediated signaling; spermatogenesis
NCBI Summary:	binds calcium ions; induces increased activity of the sarcoplasmic reticulum calcium-transporting ATPase [RGD, Feb 2006]
UniProt Code:	Q03336
NCBI GenInfo Identifier:	13928740
NCBI Gene ID:	25106
NCBI Accession:	NP_113734.1
UniProt Secondary Accession:	Q03336,Q63496, Q925W3, Q9QWP2,
UniProt Related Accession:	Q03336
Molecular Weight:	35.4 kDa
NCBI Full Name:	regucalcin
NCBI Synonym Full Names:	regucalcin (senescence marker protein-30)
NCBI Official Symbol:	Rgn
NCBI Official Synonym Symbols:	Rc; GNL; Reguc
NCBI Protein Information:	regucalcin
UniProt Protein Name:	Regucalcin
UniProt Synonym Protein Names:	Gluconolactonase (EC:3.1.1.17); GNL; Senescence marker protein 30; SMP-30
Protein Family:	Regucalcin
UniProt Gene Name:	Rgn
UniProt Entry Name:	RGN_RAT