rGFP Recombinant Protein (RPPB3401)
- SKU:
- RPPB3401
- Product Type:
- Recombinant Protein
- Uniprot:
- P42212
Description
| Product Name: | rGFP Recombinant Protein |
| Product Code: | RPPB3401 |
| Size: | 50µg |
| Target: | rGFP |
| Synonyms: | Green fluorescent protein, GFP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered yellowish solution. |
| Formulation: | The Green Fluorescent Protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8 and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPEGYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLADHYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK |
rGFP, also known as Green Fluorescent Protein, is a protein produced by the jellyfish (Aequorea Victoria) that produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag. rGFP is expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. Green Fluorescent Protein permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
rGFP Aequorea victoria produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-238 a.a.) and having a molecular mass of 26.8 kDa.rGFP is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P42212 |
| NCBI GenInfo Identifier: | 1169893 |
| NCBI Gene ID: | |
| NCBI Accession: | P42212.1 |
| UniProt Secondary Accession: | P42212,Q17104, Q27903, Q93125 |
| UniProt Related Accession: | P42212 |
| Molecular Weight: | |
| NCBI Full Name: | Green fluorescent protein |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | |
| UniProt Protein Name: | Green fluorescent protein |
| UniProt Synonym Protein Names: | |
| Protein Family: | Green fluorescent protein |
| UniProt Gene Name: | GFP�� |
| UniProt Entry Name: | GFP_AEQVI |
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