Product Name:	RFX3 Transcription Factor Activity Assay
Product Code:	TFAB00157
Target:	RFX3
Reactivity:	Human, Mouse, Rat
Sample Types:	Nuclear or cell lysates

The Assay Genie RFX3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:	4.5 hours
Detection Method:	Colorimetric 450 nm
Size:	12 x 8-Well Microstrips
Storage:	4°C for 6 months

UniProt Protein Function:	RFX3: Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left- right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells. Belongs to the RFX family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; DNA-binding Chromosomal Location of Human Ortholog: 9p24.2 Cellular Component: nuclear chromatin; nucleus; transcription factor complex Molecular Function:DNA binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding Biological Process: cell maturation; cilium biogenesis; endocrine pancreas development; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of insulin secretion; regulation of transcription, DNA-dependent
NCBI Summary:	This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]
UniProt Code:	P48380
NCBI GenInfo Identifier:	32172437
NCBI Gene ID:	5991
NCBI Accession:	P48380.2
UniProt Secondary Accession:	P48380,Q5JTL7, Q5JTL8, Q6NW13, Q8WTU4, Q95HL5, Q95HL6 A8K0H5, D3DRH8, D3DRH9,
UniProt Related Accession:	P48380
Molecular Weight:	45,715 Da
NCBI Full Name:	Transcription factor RFX3
NCBI Synonym Full Names:	regulatory factor X3
NCBI Official Symbol:	RFX3
NCBI Protein Information:	transcription factor RFX3
UniProt Protein Name:	Transcription factor RFX3
UniProt Synonym Protein Names:	Regulatory factor X 3
Protein Family:	Transcription factor
UniProt Gene Name:	RFX3
UniProt Entry Name:	RFX3_HUMAN