RFNG Antibody (PACO62839)
- SKU:
- PACO62839
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | RFNG Antibody (PACO62839) |
| Antibody SKU: | PACO62839 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:500-1:1000 |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant Human Beta-1,3-N-acetylglucosaminyltransferase radical fringe protein (71-331AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, PC3 whole cell lysate, Rat brain tissue. All lanes: RFNG antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37 kDa. Observed band size: 37 kDa. |
 | IHC image of PACO62839 diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis (By similarity). |
| Synonyms: | Beta-1,3-N-acetylglucosaminyltransferase radical fringe (EC 2.4.1.222) (O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase), RFNG |
| UniProt Protein Function: | RFNG: Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis. Belongs to the glycosyltransferase 31 family.Protein type: Motility/polarity/chemotaxis; EC 2.4.1.222; Transferase; Membrane protein, integralChromosomal Location of Human Ortholog: 17q25 |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9Y644 |
| NCBI GenInfo Identifier: | 296452991 |
| NCBI Gene ID: | 5986 |
| NCBI Accession: | Q9Y644.3 |
| UniProt Secondary Accession: | Q9Y644,O00588 |
| UniProt Related Accession: | Q9Y644 |
| Molecular Weight: | 36,424 Da |
| NCBI Full Name: | Beta-1,3-N-acetylglucosaminyltransferase radical fringe |
| NCBI Synonym Full Names: | RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| NCBI Official Symbol: | RFNG |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | beta-1,3-N-acetylglucosaminyltransferase radical fringe |
| UniProt Protein Name: | Beta-1,3-N-acetylglucosaminyltransferase radical fringe |
| UniProt Synonym Protein Names: | O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Protein Family: | Beta-1,3-N-acetylglucosaminyltransferase radical fringe |
| UniProt Gene Name: | RFNG |
| UniProt Entry Name: | RFNG_HUMAN |
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