REPS1 Antibody (PACO58432)
- SKU:
- PACO58432
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | REPS1 Antibody (PACO58432) |
| Antibody SKU: | PACO58432 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human RalBP1-associated Eps domain-containing protein 1 protein (105-230AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO58432 diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO58432 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO58432 diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | May coordinate the cellular actions of activated EGF receptors and Ral-GTPases. |
| Synonyms: | RalBP1-associated Eps domain-containing protein 1 (RalBP1-interacting protein 1), REPS1 |
| UniProt Protein Function: | May coordinate the cellular actions of activated EGF receptors and Ral-GTPases. |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a signaling adaptor protein with two EH domains that interacts with proteins that participate in signaling, endocytosis and cytoskeletal changes. The encoded protein has been found in association with intersectin 1 and Src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1 when intersectin 1 was isolated from clathrin-coated pits. The encoded protein has also been shown to interact with amphiphysin, a cytoplasmic protein at the surface of synaptic vesicles. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014] |
| UniProt Code: | Q96D71 |
| NCBI GenInfo Identifier: | 190886437 |
| NCBI Gene ID: | 85021 |
| NCBI Accession: | NP_001122089.1 |
| UniProt Secondary Accession: | Q96D71,Q5JWJ5, Q5JWJ6, Q5JWJ7, Q8NDR7, Q8WU62, Q9BXY9 B7ZBZ8, B7ZBZ9, B7ZC00 |
| UniProt Related Accession: | Q96D71 |
| Molecular Weight: | 80 KD |
| NCBI Full Name: | ralBP1-associated Eps domain-containing protein 1 isoform b |
| NCBI Synonym Full Names: | RALBP1 associated Eps domain containing 1 |
| NCBI Official Symbol: | REPS1 |
| NCBI Official Synonym Symbols: | NBIA7; RALBP1 |
| NCBI Protein Information: | ralBP1-associated Eps domain-containing protein 1 |
| UniProt Protein Name: | RalBP1-associated Eps domain-containing protein 1 |
| UniProt Synonym Protein Names: | RalBP1-interacting protein 1 |
| Protein Family: | RalBP1-associated Eps domain-containing protein |
| UniProt Gene Name: | REPS1 |
| UniProt Entry Name: |
| Antibodies |
| REPS1 Antibody (PACO05557) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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