Description
Antibody Name: | Ren1 Antibody (PACO64491) |
Antibody SKU: | PACO64491 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | WB:1:1000-1:5000, IHC:1:100-1:500 |
Species Reactivity: | Rat |
Immunogen: | Recombinant Rat Ren1 protein (65-264AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Antigen Affinity purified & Affinity purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Rat Heart tissue, Rat Liver tissue. All lanes: Ren1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa. | |
IHC image of PACO64491 diluted at 1:100 and staining in paraffin-embedded rat kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
Background: | Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. |
Synonyms: | Renin (EC 3.4.23.15) (Angiotensinogenase), Ren1, Ren |
UniProt Protein Function: | REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 3.4.23.15; Secreted, signal peptide; Secreted; Protease Cellular Component: extracellular space; membrane; cytoplasm; intracellular Molecular Function:peptidase activity; insulin-like growth factor receptor binding; endopeptidase activity; aspartic-type endopeptidase activity; receptor binding Biological Process: response to drug; response to cAMP; establishment of blood-nerve barrier; hormone-mediated signaling; drinking behavior; renin-angiotensin regulation of aldosterone production; cell maturation; male gonad development; response to lipopolysaccharide; proteolysis; angiotensin maturation; response to organic substance; beta-amyloid metabolic process; regulation of MAPKKK cascade; regulation of blood pressure; renin-angiotensin regulation of blood volume; mesonephros development; kidney development |
NCBI Summary: | catalyzes the cleavage of a Leu-Xaa bond in angiotensinogen to generate angiotensin [RGD, Feb 2006] |
UniProt Code: | P08424 |
NCBI GenInfo Identifier: | 148747255 |
NCBI Gene ID: | 24715 |
NCBI Accession: | NP_036774.4 |
UniProt Secondary Accession: | P08424,Q63497, |
UniProt Related Accession: | P08424 |
Molecular Weight: | |
NCBI Full Name: | renin |
NCBI Synonym Full Names: | renin |
NCBI Official Symbol: | Ren |
NCBI Official Synonym Symbols: | Ren1; RENAA; RATRENAA |
NCBI Protein Information: | renin; angiotensinogenase; renin 1 structural |
UniProt Protein Name: | Renin |
UniProt Synonym Protein Names: | Angiotensinogenase |
Protein Family: | Protein |
UniProt Gene Name: | Ren1 |
UniProt Entry Name: | RENI_RAT |
ELISA Kits |
Rat REN (Renin) ELISA Kit (RTFI01481) |
Rat REN (Renin) CLIA Kit (RTES00023) |
Rat Renin (Ren1) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |