Reduced Glutathione (GSH) Assay Kit - Colorimetric (MAES0042)
- SKU:
- MAES0042
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Cell Culture Supernatant, Tissue, Cells
- Sensitivity:
- 2 umol/L
- Range:
- 2-100 umol/L
- Assay Time:
- 25 min
- Shipping:
- Gel Pack
- Research Area:
- Oxidative Stress
- Research Area:
- Antioxidant
- Research Area:
- Diabetes & Obesity
Description
| Product Name: | Reduced Glutathione (GSH) Assay Kit - Colorimetric |
| Product Code: | MAES0042 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, cell culture supernatant, tissue, cells |
| Assay Time: | 25 min |
| Instrument: | Microplate reader |
| Sensitivity: | 2 µmol/L |
| Detection Range: | 2-100 µmol/L |
| Recovery Rate: | 96 |
| Inter CV: | 3.2 |
| Inter CV: | 1.9 |
Reduced glutathione (GSH) is a kind of low molecular scavenger, which can remove O2-, H2O2, LOOH. GSH is a small molecule peptide which composed of glutamic acid, glycine and cysteine, and it is the main thiol compound of non-protein in the organization. GSH is the substrate of GSH-Px and GSH-ST which is indispensable for decomposing hydrogen peroxide of these two enzymes, and it can stabilize the enzyme containing thiol and prevent hemoglobin and other auxiliary factors from the oxidative damage. Recently, it is proved that GSH is also involved in the recovery of VE to the reduction state. When lacking or depletion of GSH, it may cause producing toxic effects or increasing the toxic effects of many chemicals or environmental factors. It may be related to the increase of oxidative damage, so the amount of GSH is a vital factor to measure the body's antioxidant ability. GSH plays an important role in the researches process of prevention, recovery and treatment of atherosclerosis, coronary heart disease, anti-aging, anti-tumor, prevention, prevention of Alzheimer's disease and other diseases.
Reduced glutathione (GSH) can react with dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.
| Shelf Life: | 6 Months |
| Storage: | 2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Oxidative stress |
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